Cationic polymeric welding sweater materials and cell-penetrating peptides (CPPs) were often used as the delivery vectors in the evaluation of nucleic acid therapeutics.10-23 DNAzyme is a kind of potential antisense therapeutics by catalytic cleavage of the disease-related RNAs.Here, lipofectamine 2000 and Tat peptide were evaluated for their effect on the catalytic activity of 10-23 DNAzyme, with the observed rate constant, thermal stability, CD spectra, and PAGE analysis, with a duplex DNA mimicking DNAzyme-substrate as a control.It was shown that the cationic carriers had a negative effect on the catalytic performance of the 10-23 DNAzyme.
Significantly, the destabilizing effect of the cationic bempresas.com carriers on the duplex formation was noteworthy, as a duplex formation is an essential prerequisite in the silencing mechanisms of antisense and RNAi.